Leica confocal microscope user manual

The microscopy in medicine core mim core at emory university is a core facility providing access to histology and stateoftheart imaging instrumentation on a shareduse basis to faculty, staff and students of the emory division of cardiology and emory department of medicine. On the right hand site under the table are three buttons marked pc microscope, scanner. Whether you need an entrylevel solution or an advanced analysis and documentation package, leica microsystems provides ergonomicallydesigned manual stereo microscopes for routine tasks. Leica dm ire2 inverted leica dm rxa2 upright lasers. Instructions, manuals, brochures and catalogues if you buy a used microscope, it probably wont be supplied with an instruction manual, and most manufacturers dont keep copies of manuals for discontinued microscopes.

An introduction from your bosssupervisor is not enough the system has 5 detectors. Green confocal control switches found on the right side of the desk from left to right pc microscope, scanner power and laser power turn the laser emission key to the on position 3. Leica dm750 user manual safety regulations 8 important notes description the leica dm750 microscope meets todays state of the art of technology. Olympus ix70 inverted fluorescent microscope manuals. Use windex and cotton balls or kimwipes to clean coverslips. Tltransmitted light 2 toggles the shutter between open and closed for brightfield illumination. Fluorescence lifetime gated detection or imaging is available. Leica tcs sp5 user manual national university of singapore.

Page 1 training guide for leica sp8 confocalmultiphoton microscope las af v3. Instructions for using leica dcm3d confocal microscope. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. Microscopy in medicine lab emory school of medicine.

Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Optical sectioning and confocal microscopy kurt thorn. From basic documentation to advanced life science research las x. Leica sp2 aobs confocal microscope microscopes, imagers. Use this option to select the leica microscope you are using. A high sensitivity, inverted, pointscanning confocal system. Leica mc170 hd or leica mc190 hd user manual general notes 5 general notes safety concept before using your leica microscope camera for the first time, please read the safety concept booklet included with your instrument. Remember to activate the lasers wwl and blue diode under. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. For true confocal microscopy, you always want to choose airy 1. Leica dmre microscope photometrics coolsnap ccd fx camera imaging options. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging.

Two of these detectors are very sensitive hyd sp gaaspdetectors. If the heating system is on it means a user needs it later in the day, so do not turn it off. Quick operation manual for leica sp5 confocalmultiphoton. On the touchscreen display panel of the leica dmi8 microscope, push magnification icon 1 in figure 1 to open the control panel.

Leica sp8 confocal, sted, flim system microscopy and. There is currently no extra training fee for this microscope, however, that may change in the future. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. The book usually introduces a set of steps to accomplish a particular task with a distinctive heading. Leica sp5 scanning confocal cdb equipment resource. Autoexposure changes the detector gain values so something is visible. Fine adjustments may be made using the clickable wheel. Leica dm2500 upright fluorescent microscope manuals cell. Buy a microscope leica microscopes microscope central. Starting the leica sp5 confocal university of leicester. Interlocks are in place to prevent users being exposed directly to the beam through the eyepieces. If you need to have the scan parameters you will subsequently adjust to be in effect you.

Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. Light microscopy microscopy um biomedical research. With live acquisition going, adjust the focus to the top of the stack. Bright field, dic differential interference contrast, fluorescence hg lamp. Usermanual for leica sp8 confocal universitetet i bergen. Quick operation manual for leica sp5 confocalmultiphoton microscope. The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability. Leica application suite x las x is the one software platform for all leica microscopes. Raise stage to appropriate height and mount sample. Leica dmi6000 user manual march 2019 update page 2 leica dmi6000 microscope user policies. Live this was called fastxy in the 510 software constantly scans quite quickly at a fairly low resolution to allow you to make adjustments. Leica is a leader in the field of microscopy and has been providing innovative solutions to the educational market, industrial market, and clinical market since 1925.

Routine manual stereo microscopes leica microsystems. Do not dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual. Widely recognized for optical precision and innovative technology, leica microsystems is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning and superresolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. This video shows how to configure the internal laser power wavelength etc. Zeiss axio observer z1 manual single or tile imaging with zen live cell station. Microscope imaging software microscope imaging software from leica microsystems combines microscope, digital camera and accessories into one fully integrated solution. The confocal microscope room houses laser beams ranging from 402nm to 832nm the light ir, visible and uv from each is coupled by fiber optic to the confocal scanner, which moves a highly convergent low power beam across the specimen. Ultimate optical design and stability with extreme sensitivity and superb image quality.

Page 1 training guide for leica sp8 confocal multiphoton microscope las af v3. Leica sp5 confocal user manual date of first issue. Existing users are not allowed to train other users. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. Leica sp5 inverted confocal manual live cell station. Use in clean rooms the leica mc170 hd or leica mc190 hd digital. The confocal microscope system for highest demands in multi user facilities. To ensure safety, obtain optimum performance and familiarize yourself fully with this product, we recommend that you study this manual thoroughly. Imaging uv and vis fluorophores in fixed or live samples. Nevertheless, hazards may still arise during operation. Manual leica tcs sp ii university of massachusetts. Through the invention of the twinflect mirror, we turned our confocal platform leica tcssp8 into an easytouse, versatile light sheet microscope. When sample is in focus and positioned correctly switch microscope back to cs combi to begin imaging with the confocal.

Overview highspeed and highsensitivity multimodal confocal imaging with 2 pinhole sizes 25um and 40um four laser lines. Instructions for the leica sp5 ii laser scanning confocal microscope. Microscope imaging software products leica microsystems. Zeiss axio observer z1 manual single or tile imaging with zen. What should the user of the laser scanning microscope observe. Bright field, dic differential interference contrast, fluorescence hg lamp available filter cubes. Confocal laser scanning microscopy leica this presentation has been put together as a common effort of urs ziegler, anne greet bittermann, mathias hoechli.

To learn about the leica confocal software, use the book as a tutorial. Push the eye icon 2 in figure 1 to open the port for eyepieces 2. The ibif leica confocal microscope is made available on a feeforusehour basis to all users who have been trained. Make sure that your sample slides are clean and sealed. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. National chiao tung university, bio photonics lab, bypraveen kumar. Usermanual for leica sp8 confocal contact information. Leica dm2500 upright fluorescent microscope manuals. Via the microscope eyepieces, visualize your sample and adjust the focus z and stage position x,y accordingly 6. Use the confocal controller, or the microscope controller fine focusing knob. Leica sp5 general training manual sept 2010 robarts research. Turn on power to microscope power unit is located at your feet, next to the computer. This modulare product range can be customized to your needs with ergonomic accessories, special bases and extensive illumination options.

Leica sp2 aobs confocal microscope microscopes, imagers and. Before installing, operating or using the instrument, it is mandatory to read this user manual. The confocal microscope system for highest demands in multiuser facilities. Andor dragonfly spinning disk confocal microscope users. Introduction to confocal laser scanning microscopy leica.

Sp5 confocal microscope user manual, helene javot updated by mamta srivastava. User manual leica dmi6000 microscope university of arizona. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. The leica tcssp5 upright confocal laserscanning microscope is located in room 250 west. Dichroic color tab 1 allows the user to switch between brightfield bf or fluorescence fluo 2.

True confocal scanner leica tcs sp2 user manual ctac. This book also covers the way your leica confocal software and the leica microscope interact. Check to make sure antivibration table is on power switch in the back of unit 3. Place of use the leica ms5, mz6, mz75, mz95, mz125 and mzapo stereomicroscopes are intended mainly for indoor use. Leica dmi1 manuals and user guides, microscope manuals. Remove the dust cover from the microscope turn on the mercury lamp if you need to see the fluorescence through the eye pieces. How to use leica confocal microscope sp5 external laser.

Microscope central is proud to offer an extensive line of leica microscopes, cameras, and accessories. Log into user profile press ctrl, alt and delete simultaneously enter userid and password 5. With the leica tcs sp8 dls digital lightsheet you get a full confocal with gentle single plane illumination in one microscope. Many pages are copied from internet web pages or from presentations given by leica, zeiss and other companies. Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. Pressing autoexposure is a good starting point to get an image on the screen to optimize. Users manual fluoview fv confocal laser scanning biological microscope hardware petition this users manual is for the olympus fluoview fv confocal laser scanning biological microscope. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. Leica microscopes are trusted by teachers, lab managers, and quality control. Reserve the leica sp2 aobs confocal microscope read the user manual. In addition, this operating manual provides a rough overview of the. Zeiss 780 upright confocal manual duke light microscopy. It is a bundle of a confocal or multiphoton microscope from leica.

Training manual leica tcs sp5 confocal microscope september 2010. Enter you name, the date, the time, and the account number in the user log book. Be aware, when you switch between the dry and oilwater objectives, you need to push the objective icon. Image window robarts research institute leica sp5 training manual september 2010. Turn on pmt for detection and select lut look up table or color for that particular channel. Leica tcs sp5 confocal laser scanning microscope user guide. How to use leica confocal microscope sp5 external laser part1. It contains additional information on handling and care. Training guide for leica sp8 baylor college of medicine. User guide to the ibif leica tcs sp8 mp confocal microscope.

Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 116 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. When you hit airy 1, the red slider will adjust the pinhole to the appropriate diameter based on the objective and laser wavelengths you are using. This screen highlights the aperture opening and the intensity of the transmitted light. User manuals, guides and specifications for your leica dmi1 microscope. This microscope can accommodate specimens labeled with all fluorophores including ultra violet dapi. Database contains 3 leica dmi1 manuals available for free online viewing or downloading in pdf. Through the invention of the twinflect mirror, we turned our confocal platform leica tcssp8 into. Choose the objective 3 in figure 1 you want to use. This user manual specifies names of products or services that are trademarks or registered. Instruction manual leica dmre fluorescence microscope.

Confocal microscopy facility schepens eye research. With an intuitive user interface and straightforward navigation, it guides the user through any workflow, whether fast image acquisition or sophisticated expert analysis. Leica sp5 inverted confocal manual duke light microscopy. Andor dragonfly spinning disk confocal microscope users manual.